simple pci imaging software (Hamamatsu)
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Simple Pci Imaging Software, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simple+pci+imaging+software/simple+pci+imaging+software/pmc10411948-99-8-10
Average 90 stars, based on 1 article reviews
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1) Product Images from "Sildenafil stimulates human trophoblast invasion through nitric oxide and guanosine 3′,5′-cyclic monophosphate signaling"
Article Title: Sildenafil stimulates human trophoblast invasion through nitric oxide and guanosine 3′,5′-cyclic monophosphate signaling
Journal: Fertility and sterility
doi: 10.1016/j.fertnstert.2015.02.025
Figure Legend Snippet: Trophoblast invasion from first-trimester chorionic villous explants. First-trimester villous explants were cultured on Matrigel-coated transwell inserts and treated with vehicle or 350 ng/mL sildenafil for 72 hours before fixation. Examples of outgrowths from villous explants cultured with vehicle (A, C, E) or 350 ng/mL sildenafil (B, E, F) are shown. Bright-field images (A, B) and paired fields labeled with 6-diamino-2-phenylindole (C, D) or anti-Ki67 (E, F) are included. Size bar in (A) applies to all image panels. Outgrowth length and cell proliferation were measured using Simple PCI image analysis software (G, H). The graph in (G) shows outgrowth lengths for triplicate experiments in which 10 villous explants were measured for each treatment. * P<.05 vs. vehicle. The graph in (H) shows the percentage of cell nuclei in the outgrowth regions that were labeled with anti-Ki67 antibody. The differences between vehicle- and sildenafil-treated explants were not significant (n = 8). Error bars represent SEM.
Techniques Used: Cell Culture, Labeling, Software
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Imaging:Article Title: Sildenafil stimulates human trophoblast invasion through nitric oxide and guanosine 3′,5′-cyclic monophosphate signaling Article Snippet: Staining was imaged using a Leica DM IRB epifluorescence microscope, and images were captured using a Hamamatsu Orca digital camera. .. Images were processed to determine gray level using Simple PCI (Hamamatsu) Article Title: Sildenafil stimulates human trophoblast invasion through nitric oxide and guanosine 3′,5′-cyclic monophosphate signaling Article Snippet: Extravillous trophoblast migration was determined for each villous tip from digital images captured using a Leica DM IRB epifluorescence microscope, and a Hamamatsu Orca digital camera. .. Trophoblast outgrowth length from the villous tip at the point of initial attachment to the distal point of migration was measured using Simple PCI (Hamamatsu) Article Title: UmTea1, a Kelch and BAR domain-containing protein, acts at the cell cortex to regulate cell morphogenesis in the dimorphic fungus Ustilago maydis. Article Snippet: .. Cells were observed with Nomarski interference and epifluorescence optics using a BX61 Olympus microscope equipped with an ORCA CCD camera (Hamamatsu) and images captured using Article Title: Commonly used fertility drugs, a diet supplement, and stress force AMPK-dependent block of stemness and development in cultured mammalian embryos Article Snippet: Fluorescent antibody labeling was imaged using a Hamamatsu Orca cooled-chip digital camera and a Leica DM IRB microscope with filter sets for DAPI, FITC, and Texas Red. .. Embryos were imaged at an objective magnification of ×20 and an exposure time of 2.0 s. The FITC or Texas Red stain intensities were quantified using Simple PCI (Hamamatsu) Article Title: NIFEDIPINE PREVENTS APOPTOSIS OF ALCOHOL-EXPOSED FIRST TRIMESTER TROPHOBLAST CELLS Article Snippet: Images were taken with a Leica (Wetzlar, Germany) DM IRB epifluoresence microscope interfaced with a Hamamatsu Orca Digital camera (Hamamatsu City, Japan). .. Fluorescence intensities were analyzed using Article Title: Enhancement of trophoblast differentiation and survival by low molecular weight heparin requires heparin-binding EGF-like growth factor Article Snippet: Streams of migrating extravillous trophoblast cells were measured at the tip of each villous explant using digital images captured with a Hamamatsu (Hamamatsu City, Japan) Orca digital camera mounted on a Leica (Wetzlar, Germany) DM IRB epifluorescence microscope. .. Outgrowth length was measured using Simple PCI (Hamamatsu) Article Title: Mitochondrial stress restores the heat shock response and prevents proteostasis collapse during aging Article Snippet: .. Motility videos were captured using a Leica stereomicroscope at 10X magnification with a Hamamatsu Orca-R2 digital camera C10600-10B and Hamamatsu Article Title: Rosiglitazone blocks first trimester in-vitro placental injury caused by NF-κB-mediated inflammation Article Snippet: .. Using Software:Article Title: Sildenafil stimulates human trophoblast invasion through nitric oxide and guanosine 3′,5′-cyclic monophosphate signaling Article Snippet: Staining was imaged using a Leica DM IRB epifluorescence microscope, and images were captured using a Hamamatsu Orca digital camera. .. Images were processed to determine gray level using Simple PCI (Hamamatsu) Article Title: Sildenafil stimulates human trophoblast invasion through nitric oxide and guanosine 3′,5′-cyclic monophosphate signaling Article Snippet: Extravillous trophoblast migration was determined for each villous tip from digital images captured using a Leica DM IRB epifluorescence microscope, and a Hamamatsu Orca digital camera. .. Trophoblast outgrowth length from the villous tip at the point of initial attachment to the distal point of migration was measured using Simple PCI (Hamamatsu) Article Title: UmTea1, a Kelch and BAR domain-containing protein, acts at the cell cortex to regulate cell morphogenesis in the dimorphic fungus Ustilago maydis. Article Snippet: .. 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Fluorescence intensities were analyzed using Article Title: Enhancement of trophoblast differentiation and survival by low molecular weight heparin requires heparin-binding EGF-like growth factor Article Snippet: Streams of migrating extravillous trophoblast cells were measured at the tip of each villous explant using digital images captured with a Hamamatsu (Hamamatsu City, Japan) Orca digital camera mounted on a Leica (Wetzlar, Germany) DM IRB epifluorescence microscope. .. Outgrowth length was measured using Simple PCI (Hamamatsu) Article Title: Mitochondrial stress restores the heat shock response and prevents proteostasis collapse during aging Article Snippet: .. Motility videos were captured using a Leica stereomicroscope at 10X magnification with a Hamamatsu Orca-R2 digital camera C10600-10B and Hamamatsu Article Title: Rosiglitazone blocks first trimester in-vitro placental injury caused by NF-κB-mediated inflammation Article Snippet: .. Using Migration:Article Title: Sildenafil stimulates human trophoblast invasion through nitric oxide and guanosine 3′,5′-cyclic monophosphate signaling Article Snippet: Extravillous trophoblast migration was determined for each villous tip from digital images captured using a Leica DM IRB epifluorescence microscope, and a Hamamatsu Orca digital camera. .. Trophoblast outgrowth length from the villous tip at the point of initial attachment to the distal point of migration was measured using Simple PCI (Hamamatsu) Microscopy:Article Title: UmTea1, a Kelch and BAR domain-containing protein, acts at the cell cortex to regulate cell morphogenesis in the dimorphic fungus Ustilago maydis. Article Snippet: .. Cells were observed with Nomarski interference and epifluorescence optics using a BX61 Olympus microscope equipped with an ORCA CCD camera (Hamamatsu) and images captured using Staining:Article Title: Commonly used fertility drugs, a diet supplement, and stress force AMPK-dependent block of stemness and development in cultured mammalian embryos Article Snippet: Fluorescent antibody labeling was imaged using a Hamamatsu Orca cooled-chip digital camera and a Leica DM IRB microscope with filter sets for DAPI, FITC, and Texas Red. .. Embryos were imaged at an objective magnification of ×20 and an exposure time of 2.0 s. The FITC or Texas Red stain intensities were quantified using Simple PCI (Hamamatsu) Fluorescence:Article Title: NIFEDIPINE PREVENTS APOPTOSIS OF ALCOHOL-EXPOSED FIRST TRIMESTER TROPHOBLAST CELLS Article Snippet: Images were taken with a Leica (Wetzlar, Germany) DM IRB epifluoresence microscope interfaced with a Hamamatsu Orca Digital camera (Hamamatsu City, Japan). .. Fluorescence intensities were analyzed using |
